本文主要介紹了貝塞爾光束達(dá)曼光柵調(diào)制柵格場(chǎng)在側(cè)向片層熒光顯微領(lǐng)域的應(yīng)用。該技術(shù)主要應(yīng)用在生物領(lǐng)域。 A.c NOous|
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Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools.We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic s y>}2orj~
instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems. dU&hM<.|
